Astatine-211 labelled monoclonal antibodies as cell-specific cytotoxic reagents. by Wendy J. Bateman

Cover of: Astatine-211 labelled monoclonal antibodies as cell-specific cytotoxic reagents. | Wendy J. Bateman

Published by University of Birmingham in Birmingham .

Written in English

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Thesis (Ph.D.) - University of Birmingham, Dept of Immunology.

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Open LibraryOL13825141M

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Monoclonal antibodies were radiohalogenated with the intermediate reagent N -succinimidyl 3-(trimethylstannyl)benzoate and characterized in terms of radiochemical yield and in vitro binding properties. In vitro OVCAR-3 cells were irradiated using an external Cobalt beam, as reference, or Atalbumin and labeled by:   EXPERIMENTAL DESIGN: At was produced by cyclotron irradiation of a bismuth target and isolated using a novel dry distillation procedure.

Monoclonal antibodies were radiohalogenated with the intermediate reagent N-succinimidyl 3- (trimethylstannyl)benzoate Cited by: Monoclonal antibodies were radiohalogenated with the intermediate reagent N-succinimidyl 3-(trimethylstannyl)benzoate and characterized in terms of radiochemical yield and in vitro binding properties.

In vitro OVCAR-3 cells were irradiated using an external Cobalt beam, as reference, or Atalbumin and labeled antibody. As part of an ongoing phase 1 study, we examined the effect of radioimmunotherapy with ¹³¹I-labeled B-cell-specific Astatine-211 labelled monoclonal antibodies as cell-specific cytotoxic reagents.

book monoclonal antibody in 10 patients with CDpositive B-cell. Abstract. The objective of this study was to translate reaction conditions and quality control methods used for production of an astatine( At)-labeled anti-CD45 monoclonal antibody (MAb) Author: Li, Yawen.

Radionuclides that decay by the emission of alpha-particles such as the heavy halogen astatine (At) offer the exciting prospect of combining cell-specific molecular targets with radiation.

In order to specifically irradiate the tumor cells At was labeled to monoclonal antibodies, targeting certain cell surface epitopes. The PhD thesis, presented on pagesAuthor: Jörgen Elgqvist.

In this study, we have investigated whether hyperthermia increased the cytotoxicity of an At-labelled monoclonal antibody (MAb) in tumour spheroids with a radius (approximately microm).

Astatination of the antibody was conducted essentially as previously described. mAbs were labeled with At using the labeling reagent m-MeATE [N-succinimidyl 3. In this study, we have investigated whether hyperthermia increased the cytotoxicity of an At-labelled monoclonal antibody (MAb) in tumour spheroids with a radius (approximately microm) greater.

Astatinelabeled BC8-B10 monoclonal antibody is a monoclonal antibody, called anti-CD45 monoclonal antibody BC8-B10, linked to a radioactive/toxic agent called astatine Anti-CD45 monoclonal antibody BC8-B10 is attached to CD45 antigen positive cancer cells in a targeted way and delivers astatine.

Astatinelabeled BC8-B10 monoclonal antibody is a monoclonal antibody, called anti-CD45 monoclonal antibody BC8-B10, linked to a radioactive / toxic agent called astatine Anti-CD45 monoclonal antibody BC8-B10 is attached to CD45 antigen positive cancer cells in a targeted way and delivers astatine.

Astatine conjugated to an anti-CD20 monoclonal antibody eradicates disseminated B-cell lymphoma in a mouse model At-labeled 1F5 monoclonal antibody (mAb) (anti-CD20) was studied in both bulky lymphoma tumor xenograft and MRD Bifunctional decaborate (BNCS) reagent.

A non-cytotoxic antibody BK was rendered cytotoxic by coupling the antibody to the radioisotope astatine BK was chosen for this study since this antibody recognises an antigen which is uniformly distributed on human bone marrow leukocytes.

Hence the ability of astatine labelled antibodies. Astatine At anti-CD45 monoclonal antibody BC8-B10 binds to CD45 antigen, a receptor protein-tyrosine phosphatase expressed on the surface of both normal and malignant hematopoietic cells.

After binding and internalization by CDexpressing tumor cells, this agent may deliver a cytotoxic dose of alpha radiation.

Highly localized radiotherapy with radionuclides is a commonly used treatment modality for patients with unresectable solid tumors. Herein, we propose a novel α-nanobrachytherapy approach for selective therapy of human epidermal growth factor receptor 2 (HER2)-positive breast cancer.

This uses local intratumoral injection of 5-nm-diameter gold nanoparticles (AuNPs) labeled. Title: AstatineLabeled Radiotherapeutics An Emerging Approach to Targeted Alpha-Particle Radiotherapy VOLUME: 6 ISSUE: 14 Author(s):Michael R.

Zalutsky and Ganesan Vaidyanathan Affiliation:Department of Radiology, BoxDurham, NC USA Keywords:Astatine labeled. Larsen RH, Bruland OS, Hoff P, et al.

Inactivation of human osteosarcoma cells in vitro by At-TP-3 monoclonal antibody: Comparison with astatinelabeled bovine serum albumin, free astatine. Antibody−drug conjugates (ADCs) combine the specificity of monoclonal antibodies (mAbs) with the potency of cytotoxic molecules, thereby taking advantage of the best characteristics.

A system and method for automatic production of astatine labeled molecules is described. The invention represents a significant advantage in the preparation of At radiopharmaceuticals. Monoclonal antibody and F(ab′) 2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration.

Cancer Res. ; – Zalutsky MR, Vaidyanathan G. Astatinelabeled. Chemical methods for the production of astatine labeled radiophrmaceuticals Value Proposition Alpha particles can focus large amount of energy over a few cell diameters allowing for targeted tumor.

In this study, a pretargeting model system of avidin‐conjugated monoclonal antibody trastuzumab and biotinylated, At‐labeled poly‐L‐lysine was constructed and analyzed in vitro. METHODS: Avidin.

The cells were labeled with ATP Synthase Subunit beta (3D5AB1) Mouse Monoclonal Antibody (Product # A) at 2 µg/mL in % BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody.

Ceriani, R. and Blank, E. Experimental therapy of human breast tumours with labelled monoclonal antibodies prepared against the human milk fat globule. Cancer Res., 48, –. The objective of this study was to translate reaction conditions and quality control methods used for production of an astatine( At)-labeled anti-CD45 monoclonal antibody (MAb) conjugate.

To evaluate this hypothesis, ()At-labeled 1F5 monoclonal antibody (mAb) (anti-CD20) was studied in both bulky lymphoma tumor xenograft and MRD animal models.

Superior treatment responses to ()At-labeled. This is a list of therapeutic, diagnostic and preventive monoclonal antibodies, antibodies that are clones of a single parent used as drugs, the International Nonproprietary Names (INNs) end in -mab.

The remaining syllables of the INNs, as well as the column Source, are explained in Nomenclature of monoclonal antibodies. A monoclonal antibody (mAb or moAb) is an antibody made by cloning a unique white blood subsequent antibodies derived this way trace back to a unique parent cell.

Monoclonal antibodies can have monovalent affinity, binding only to the same epitope (the part of an antigen that is recognized by the antibody). In contrast, polyclonal antibodies.

Product # Description. Clone. Application. Species Reactivity. Image. Add to Cart. WHM2: Monoclonal Anti-ACTA2 antibody produced in mouse: 4AH3, monoclonal. Generating Monoclonal Antibodies Edward A. Greenfield Dana-Farber Cancer Institute, Boston, Massachusetts monoclonal antibod-ies are powerful reagents for testing for the presence of a desired epitope.

Hybridoma cell lines also Immunoassays with labeled antibody. Monoclonal antibodies are antibodies that are made by identical immune cells that are all clones of a unique parent cell and bind to the same almost any substance, it is possible to produce monoclonal antibodies.

The antigen in the specimen and an enzyme-labeled antigen compete for binding sites on reagent antibody. When enzyme-labeled antigen binds to antibody, enzyme activity is inhibited. Enzyme. Secondary Antibodies are primarily utilized for amplification of the antibody/antigen reaction.

Control Reagents are well-suited as qualitative negative controls for the primary antibodies. All antibody reagents. After activation process, pipet 20 µL of monoclonal antibody into a labeled tube. Add Monoclonal Antibodies Source Book, SectionCHARACTERIZATION To ensure consistently high-quality reagents, each lot of antibody.

Virtually all MM clones are exquisitely sensitive to radiation, and the α-emitter astatine ( At) deposits prodigious energy within 3 cell diameters, which is ideal for eliminating MRD if effectively targeted.

CD38 is a proven MM target, and we conjugated At to an anti-CD38 monoclonal antibody. Prepare appropriate dilution of primary antibody with antibody dilution buffer. Add μl to wells and incubate at 37°C for 1 hr.

Wash three times with wash buffer. Add 67 ng/well DELFIA Europium-labeled Anti-mouse IgG or Anti-rabbit IgG, diluted in μl/well antibody. Radioactive Monoclonal Antibodies Against Cell Surface Antigens for Labeling Leukocyte Subpopulations A greater localization of cytotoxic T cells than helper T cells has been observed in Peyer’s patches, but their localization is equal in peripheral nodes (7).

Ilabeled crosslinking reagent. development of reagents for protein and peptide radioiodination that decrease deiodination in vivo by up to fold, b) demonstration that At labeled monoclonal antibodies are effective in the treatment of a rat model of neoplastic meningitis, c) synthesis of a thymidine analogue labeled.

Q J Nucl Med mitomycin-phosphate by a bispecific anti-CD30/anti- – alkaline phosphatase monoclonal antibody. Cancer Res Zalutsky MR, Vaidyanathan G () Astatinelabeled. Phase I clinical trials (Sidebar ) have been performed with bismuth and astatinelabeled monoclonal antibodies in patients with leukemia and brain tumors (Couturier et al.

). Antibody-secreting B cells are immortalized by merging B lymphocytes and myeloma cells in the immunized mouse. The hybridoma cell line secreting the monoclonal antibody is then selected using limited dilution cloning. The monoclonal antibody .Monoclonal Antibodies are cells derived by cell division from a single ancestral onals are a class of antibodies with identical offspring of a hybridoma and are very specific for a particular location in the body derived from a single clone and can be grown indefinitely.

Monoclonal Antibodies .

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